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 Wysłany: Czw 8:38, 02 Gru 2010 Temat postu: MAC Mascara Anti-bacterial protein Rs-AFP2 summer |
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Anti-bacterial protein Rs-AFP2 summer directed mutation and its effect on E. coli
n2 (RS-AFP2) GeneandItsEffect0nExpressionintheE. ColiZhuJianqing, LiuZhu, HuXinwen,MAC Mascara, YangZhirong (CollegeofLifeSciences, SichuanUniversity,PUMA shoes wholesale, Chengdu610064) (\Themutant, whichincludedRs-AFPgene, wasconstructed. SequenceanalysisshowedthatthePCRproductWaS240bp. Ithadanopenreadingframe, encodingsignalpeptideof29aminoacidsandITla-turepeptideof51aminoacids. BycomparisonbetweentheRs-AFP2oneandthemutatedRs-AFPmgene, therewerefivebasesdifferencesintheLyS3aminoacid (TTTA), theGln5 (CAG A 0A), andtherearcodon6 (CAG-~ CGA). Afterremovingthesignalpeptide, thedigestedproductswereinsertedintopET a 21b (+). TheexpressionvectorswereexpressedinB1strainafterinductionwithIPTG. SoftwareanalysisshowedthatthetargetproteinofPETAFPooccupied3% andthatofmutatedPETAFPmoccupied8% inthewholebacterialprotein. ThetargetproteinWaSmainlyexistedininclusionbodies. Theinclusionbodieswerebrokeandthenre-folded. TheinhibitionexperimentsshowedthatantifungalradiusofpETAFPmWaSbiggerthanthatofpETAF-Po. ltheseshowedR-AFPmgenehadvalidlyincreasedtheexpressionvolumeincontraStwithRs-AFP2gene ・ Keywords: Raphanussativus-antifungalprotein2 (Rs-AFP2),MAC 88 color eyeshadow, Sited-directedmutagenesis,sheepskin ugg boots, CodonbiaS,PUMA trionfo baylee shoes, Expression of a 23 -
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