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 Wysłany: Wto 12:24, 08 Mar 2011 Temat postu: Ultrasonic Technology Applications in Pathology _1 |
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Ultrasonic Technology Applications in Pathology
Organization antigen. Ultrasonic fixed biopsy tissue, not only greatly reducing the fixed Ho, and organizational structure of well-preserved, producer of the fixation method than conventional better. 2 tissue dehydration, transparency, good dip wax the ultrasonic fixed tissue blocks were set for a warm acetone 5afin 7mln, look askance water in a clean, direct dipping wax, the whole process takes only a 15rain rapid production of a 20mirt, with the conventional fast Production time is not longer than, the production quality of biopsy quality is better,[link widoczny dla zalogowanych], especially the lymph nodes and brain. Lymph nodes because of organizational structure is dense, cellular components and more rapid dehydration are often not completely normal, white light microscope, stained poorly. Of diagnosis, ultrasonic rapid dehydration, only an appropriate extension of time, and we grope after that dehydration what 10rain, the temperature of 75 is more suitable. Light microscope, lymph node structure is clear. Thoroughly good dyeing effect dehydration. Brain tissue producer, taking the village as a complete, fast to avoid the conventional paraffin wax, tissue pieces, the lesions can not be completely apparent shortcomings,[link widoczny dla zalogowanych], especially for diagnosis that is. Dimethyl Ping is often used in optical microscopy of transparent agent, it has a lot of toxic, anti-carcinogenic effects of human potential. Dimethyl-ping as a intermediary, mixed it with alcohol, can dissolve paraffin. Facilitate the embedding medium of the immersion effect. The ultrasonic dehydration without xylene, can be directly dipped wax we use ultrasonic dehydration embedded transparent wax dipped throughout the dehydration process,[link widoczny dla zalogowanych], organization, and the solvent remain constant vibration, so that the solvent molecules continued to spread through the semi-permeable membrane, penetrate into the organizations to, as the constant vibration of molecules and some physical properties of the permeability change, then dip the wax can be directly embedded in the supporting role to achieve the 3 decalcified decalcified bone technology has been the problem of pathological . Application of ultrasonic technology can be demineralized bone tissue. First cut into 3era × 2cm × 1.5cm size I5 times the volume of bone tissue immersed in 10% formalin and fixed in the next five ultrasonic transit, and then placed in 4% EDTA-2Na (4gEDTA-2Na dissolved in 100ml dry distilled water) decalcification solution, the temperature maintained at 50 ~ 55 under ultrasonic decalcification 10afin, abandoned to the decalcification solution, plus one new one to determine decalcified decalcified decalcified repeated liquid air backward to complete decalcification solution, ultrasonic washing under tap water acid 5mln, following the same routine production. Rapid Decalcification with the heading Compared transition we believe that ultrasound is more convenient crossing decalcification, it is appropriate ju control, timing. Compared with conventional room temperature decalcification. Ultrasonic decalcification crossing. Over the 24h 30min reduced to as one not only shorten decalcification time. And more thorough decalcification, made of slices does not damage the blade. Light microscope. Well-preserved bone structure. Conventional HE staining 4 staining degree of vulnerability to ju, cold weather is not easy coloring, especially the beads frozen section staining, frozen sections have not been out of dehydration, Room ju stained light, the light color cell eucalyptus, cell outline was not clear. HE staining of ultrasound transition. Either paraffin or frozen sections, simply release the slices in the hematoxylin staining solution tangled. In the role of 308 ~ lmin under ultrasound to achieve the desired result. Ultrasound technology can also be used for some special staining. At present, more special staining using microwave radiation, such as the application of radiation technology for micro-PAM stain transition J. We work in the ultrasound instrument for acid-fast staining, sections after dewaxing, place a basic fuchsin carbol solution, the next transition in the role of ultrasound 2 75, tap water wash. 2o% sulfuric acid under microscope control differentiation. Washing. Dehydration were mounted. Slice a clear background, eosinophils acid red bar, made of slices with the conventional method is no different. Application of ultrasound for special dyeing. According to the required temperature for each particular dye staining transferred to appropriate what you can achieve satisfactory results. Biopsy in ultrasonic vibration of transition,[link widoczny dla zalogowanych], through cells and other substances in water,[link widoczny dla zalogowanych], resulting in molecules of these substances have high-speed operation thereby reducing the thermal effect of dyeing time, improve work efficiency. And it Anglo-free operation of the microwave dyeing carried out in the wet box-3J, disheartened by the degree difficult to grasp and other shortcomings. Ultrasound technology application in pathology, we believe that ultrasound-speed ferry in Jue producer, decalcified and so much practical, worthy of promotion.
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