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 Wysłany: Nie 6:38, 30 Sty 2011 Temat postu: uggs boots italia In vitro culture of sea buckthor |
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In vitro culture of sea buckthorn
The total amount of elements from the MS of 4530mg / L down to 1300mg / L, the cultured explants 10 ~ 15d, the observed growth of meristem activity, growth and development of explants of exogenous growth also depends on the medium factors and cytokines in the absolute concentration and balance. In the medium without any hormones in explants of 100 deaths, while in NAA0.03 ~ O. 05rag / L, KT0.3n0.5mg / L, the train 6 ~ 7d, observed in 85 explants volume increased to 10 ~ 15d when the initial leaf formation, and ultimately the formation of plantlets. With the same concentration of 6-BA instead of KT, explants can survive, but the survival rate decreased. Increased when the NAA 0.1mg / L, KT is 0.5mg / L,[link widoczny dla zalogowanych], the occurrence of the outer base of a large number of explants callus, explants do not grow and together with the browning of callus, and ultimately death. Tests showed that the growth of apical meristem requires not only low-salt medium, but also a lower level of hormone substances, hormones or hormone-free high concentration, will result in the death of explants. 34 explants containing NAA0.03 ~ 0.05mg / L,[link widoczny dla zalogowanych], KT0.3 ~ 0.5mg / L of low salt culture medium to about 30d, showing the stem continues to elongate, leaf unfolding and there are always new leaves appear at the same time observed in this medium, the differentiation of the root to form a complete regeneration, but the root differentiation was low, only 25 strains developed. To 1.0cm above the growth of small plants transferred to hormone group was divided into NAA0.002mg / L, KT1.0mg / L of the medium, after about 15d, the majority of small plants produce new roots. Seabuckthorn explants that appropriate in vitro culture medium for. MS + NAA0.03 ~ 0.5mg / L + KT0.3 ~ 05mg / L, rooting medium was MS + NAA0.002mg / L + KT1.0mg / L. 2.4 The differences between varieties of different species inoculated in the same medium, were cultured under the same conditions, found that different species in vitro and regeneration vary widely. 4 varieties were tested, Ka Niya, 90-3-2 regenerative ability, can directly and then generate a complete plant, and 90-2-1 and another male plant regeneration capacity is weak, did not form shoots. This may be due to physiological characteristics of different varieties and different content of endogenous hormones, exogenous hormones so the type and concentration requirements are different. 3 Discussion 'test results showed that in vitro methods is to achieve effective way Seabuckthorn asexual. Vitro during the course of sea buckthorn, although small plants can form a complete and propagation medium in the base of explants a small amount of callus, did not affect the growth of the top, but when hormone high Excessive growth of the callus to restrict growth of the top, which also quickly browning callus died. Stem, leaf loaded with explants cultured in vitro has also been a small amount of callus, it is difficult to differentiate, and die very quickly, showing a large number of differentiated shoots through callus way for sea buckthorn seedling propagation is still a important issue. Inoculation of different varieties in the same medium, were cultured under the same conditions and found that in vitro culture of different species and regeneration vary widely. A total of 5 varieties tested, the Katu Ni gift ,89-1-1 regeneration ability,[link widoczny dla zalogowanych], and can directly and then generate a complete plant, and H2,[link widoczny dla zalogowanych], HS-1 and another male plant's regenerative capacity is weak, did not form shoots. This may be due to physiological characteristics of different varieties and different content of endogenous hormones, exogenous hormones so the type and concentration requirements are different. Therefore, the study of different species adaptability to different media at the same time, research has wide adaptability of the medium is also necessary. 'In this study, although small plants can form a complete, and can be seen in the propagation medium on the regeneration of plants collateral production, ramet survival after independence,[link widoczny dla zalogowanych], but the speed is slow, and occurrence of collateral regeneration of plants only strain 10 or so, reproduction ratio is low, reach the purpose of propagation. To be suitable for further screening Seabuckthorn explants cultured in different medium, increase the breeding ratio to achieve the purpose of propagation. It can also be obtained directly by means of bio-technology genetic variation, and create new types.
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