veoexrmpzt
Dołączył: 21 Lut 2011
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 Wysłany: Nie 19:07, 13 Mar 2011 Temat postu: tory burch wso aor kfiq iek |
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Coxsackie virus B5 micro model of myocardial cells
Commonly used PLA: Determination of one of the Comparison of this law and Zieve , are the following advantages : (1) Zinc law terminating the anti- straight , the subject of organic solvent extraction ,[link widoczny dla zalogowanych], drying , TLC , dissolved in organic solvents Before the organic solvent with the ancient NaOH titration . Law HCI solution to terminate the reaction can be titrated directly . (2) zi method time-consuming > 2oh. Rate method less than 2h. (3) ZJ. titration with the indicator to determine eve 's end,[link widoczny dla zalogowanych], errors. Law to control tube as the standard, can be repeated high sensitivity titration , pH meter , compared with little error . (4 rate method titration with HCI ,[link widoczny dla zalogowanych], reliable and convenient than using NaOH . Vogel (1960) found that 65 ℃ water bath 75m ~ n or 90 ℃ water bath 30minPLA2 activity unchanged . Ziev ~ 55 ℃ sample report relies on a water bath pre- 30min, and then pH8 .5 O reaction,[link widoczny dla zalogowanych], free fatty acids can be completely excluded the interference of other enzymes . this law by 60 ℃ water bath 30m ~ n6 So far, all methods measured PLA2 activity in body fluids and tissues are larger 【 variation , is no exception to this law Data exclude the introduction of changes in experimental error , we first examined the determination of PLA2,[link widoczny dla zalogowanych], inter and intra variability , the results show that they are small. homogenate protein content similar variation less than 3 , indicating that cell disruption is not due to different levels of Caused by animals, so this variation is mainly the result of individual differences . alveolar epithelial type I cells of the PLA2 does not depend ca
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