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 Wysłany: Czw 6:33, 23 Gru 2010 Temat postu: stivali ugg No.813 properties of protease produced |
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No.813 properties of protease produced by
Science (Engineering Science Edition) No. 2, No. 2 substrate solution and mixed 2mi. At 40 \The results show that, NO813 proteinase on casein, collagen, bovine serum albumin. Hydrolysis of egg and so on all activity, but no hydrolysis of lysozyme. Casein which the best substrate. Wrap 3 enzyme hydrolysis of several natural protein 2.8 surfactants on the enzyme activity to take lml of enzyme solution, adding 0.03% (concentration in reaction solution) surfactant. Heat for 10 minutes, with Flo1i Determination of residual enzyme activity as follows: Wrap 4 surfactants on the enzyme activity of the above Table 4 shows that surfactants in alkaline conditions, the enzyme micro-activation, which increases activation level effect more apparent in the neutral condition is not much impact on the activity. Therefore, the application of the enzyme in the future prospects in the leather. 2.9EDTA, PMSF activity of the enzyme solution with EDTA,[link widoczny dla zalogowanych], PMSF mixed (the reaction between the solution of a concentration of 1 �� 10 M, pH9.0) at 40 * (3 Yan-Bao and other properties of protease produced by No813 111 temperature of 1O minutes. and then measure the remaining enzyme activity. Table 5. It can be seen NO813 neutral and alkaline protease produced roughly the same (neutral ƫ��), EDTA and PMSF ��not completely inhibit the enzyme activity of the arrow, note also There are some other protease. bad 5EDTA, PMSF agent on the power of the tongue Animals i u, Inl over the relative activity 4365100189843.5237054.39772.24 system that bacteria produced the protease No.813 temperature and pH of its better stability. Ca2 on The enzyme was activated for the car,[link widoczny dla zalogowanych], leather Jing common surfactants,[link widoczny dla zalogowanych], such as JFC, peregal so slight activation of casein, so the bacteria produce the protein casein prospects good in leather. References Chiu Po and so on. froming Alkaline Protease Strain. Microbiology .1988.15 (3) :101-106 Zhao Yongfang. biochemical techniques and human principles. Wu Chu: Wu Zhu University Press .1994.418 Department of Sichuan Union University Students . biochemical experimental techniques. Chengdu: Sichuan Union University, Northwest Institute of Light Industry Press .1988.220 leather leather Department of Inspection Manual Beijing: Light Industry Press. 1988,80-88 (edit Huang,[link widoczny dla zalogowanych], hfl1) StudyOUThePropertiesofProteinaseFromNo.813LiuYanXianZhangYizheng (Departmentof.LeatherEngineering . SUU) AprotelnasefromNo.813waspartiallypurifiedthroughaproce ~ involvingammoniumsulfatepreclptation.dialyze, lyopbiligation, afluffyproductwasobtained.Theoptimumpnandtemperatureforca5einhydroly grapes swere9.0and50 ��. respectively.Theer ~ zymewassta.bleoverthepnrange8tol1.Ca2 'enhancedtheheatandpHstabilityoftheenzyme.Theelxzymew �� partiallyinactiviedaftertreatmentwithEDTA.andPMSF (1 �� l0M). C,[link widoczny dla zalogowanych],. Fe2. zn2 '. C (1 �� l0 a' M) inhibitedtheenzylneactivity.whileMn2trendstostimulateactivity.Col ~ en.Bovineserllmalbumin.eggalbumin.caseinweresusceptibletoenzymatichydrolysis, butnot1ysozyme.keyWordsmsalt-enduringstrainNO813.proteinase.property a ~ a
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