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 Wysłany: Sob 12:07, 19 Mar 2011 Temat postu: Paeonol Cynanchum paniculatum Determination of _29 |
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,[link widoczny dla zalogowanych]
Cynanchum paniculatum in the Determination of paeonol
Into the liquid chromatography, determination, the amount of the reference substance for the horizontal axis,[link widoczny dla zalogowanych], the absorption peak area value of the vertical axis, the standard curve; experimental data by linear regression, the regression equation A = 4216.8m a 9.5668 (r = 0 .9999, n = 5). The results showed that: paeonol reference standard in the range of 0.0352 ~ 0.2816Ixg, the amount of peak area with the reference substance was a good linear relationship. 2,6-prepared for new stability test for the sample solution, in 0 ... 124,6,8,16,24 h contents were determined, the average content of 1,24%, RSD 0.4%. The results showed that: in the 24h test solution within the stable. 2.7 repeatability take 6 copies of the same number of samples were made by the above method for the test solution was determined Paeonol,[link widoczny dla zalogowanych], The average content of 1,24%, RSD was 0.9% (n = 6). 2,8 recoveries to take a known test paeonol content for the test product; 0,81%, calculated on dry goods to 0,94%) 5 copies, each of about 0,125 g, precision set, precision added concentration of 0.9392mg · ml control solution paeonol 1ml, after mixing, according to the test solution is made to determine Paeonol, calculations recovery. Results: The average recovery was 100,96%, RSD was 1,9% (n = 5). Table 1 2,9 recoveries results of determination and concentration to determine the limits of Cynanchum paniculatum samples taken 8 groups, respectively, determined by the above method Paeonol, the results in Table 2. Chinese Pharmacopoeia 2000 edition under Cynanchum paniculatum determined by UV spectrophotometry Paeonol, the limit is: are calculated by drying, including paeonol (C. H. 0) not less than 1.30%. The high performance liquid chromatography in 8 groups Paeonol Cynanchum paniculatum, in addition to a number of 0.94%, while other samples were more than 1.30%, so the temporary retention of the original standard limits set by drying are calculated with paeonol (C. H. 0.) of not less than 1.30% Table 2 Number determination results from the sample size paeonol origin,% 3 for the discussion of Cynanchum paniculatum Qufeng dampness, pain itching class commonly used Chinese medicine, the calendar version of the Chinese Pharmacopoeia contained in the base of the original are Luomo Ke Xu Changqing Xu Changqing dry plant roots and rhizomes. According to the literature. Records, Xu Changqing primary production in Jiangsu, Zhejiang, Anhui,[link widoczny dla zalogowanych], Shandong, Hubei, Hunan, Henan and other provinces, over more production for own consumption. Jilin, Gansu, Shandong, Fujian,[link widoczny dla zalogowanych], Zhejiang, Jiangxi, Guangxi and other places with the roots and rhizomes, mostly with other parts of the whole plant with roots. The more samples collected from the market for the whole plant with roots, and its paeonol content compared with the roots and rhizomes, in addition to a lower, the rest were higher than root and rhizome. Due to the limited samples collected, with the whole grass roots and rhizomes with roots paeonol content of the law pending further investigation. Meanwhile, the author proposed in the revised 2005 edition of Chinese Pharmacopoeia, whether full use of medical resources from the point of view, the Cynanchum paniculatum expand the base of the original band Luo Moke root of Cynanchum paniculatum plant whole plant.
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