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 Wysłany: Sob 22:05, 22 Sty 2011 Temat postu: Musk strong bone paste in camphor and methyl salic |
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Camphor and musk strong bone paste in the determination of methyl salicylate
Preparation of solution products to take appropriate reference substance camphor and methyl salicylate, precision set, made of vinegar acid ethyl ester were 10mg per 1ml and l5-ng with the solution, as a reserve liquid. Precise amount of stock solution were appropriate, precision internal standard solution 5ml, made of camphor, ethyl acetate stock solution added per lml containing 1.99mg, 2.12mg of the solution, as a control solution of camphor; stock solution of methyl salicylate Add 1ml of ethyl acetate into each containing 1.41mg, 1.55mg of the solution, as a control solution of methyl salicylate. 2.5 the standard curve were accurately weighed 3.8924g reference substance camphor and methyl salicylate and 3.7683g, respectively,[link widoczny dla zalogowanych], set l00ml flask, add ethyl acetate dissolved and diluted to the mark, made with each lml 40mg of the solution, as a reserve liquid. Stock solution were further precision of the two internal standard solution 5ml, diluted with ethyl acetate are produced per each containing 1ml (0.8,1.6,4.8,8.0,16.0,24. 0,32.0) mg of the solution, respectively, the precise amount of solution of ll, injected into the gas chromatography (GC shown in Figure 1), camphor and methyl salicylate respectively, the amount of reference substance (g) is abscissa, camphor and methyl salicylate peak area of reference substance solution and the internal standard peak area ratio of Chennai ordinate standard curve linear regression equations were calculated as follows: Y = 0.1497x a 0,0106,[link widoczny dla zalogowanych], r = Y = 0.1054x 0.9999 and a 0.0071, r = 0.9999, results showed that: camphor and methyl salicylate in (0.8-32) mg peak area within the linear relationship. 2.6 The precision of precision drawing camphor reference solution (0.08mg/m1) 1l, repeat 5 times into the sample, the measured peak area RSD was 0.61%, (n = 5) showed good precision instrument . 2.7 Stability testing of this product in the same preparation of the test solution were placed after the 0, l, 2,4,6,8 h, respectively, precision drawing ll, were injected into the gas chromatography determination of camphor, RSD = 1. 02% (n = 6), methyl salicylate RSD = 1.13%, (n = 6) showed that the test of the product liquid methyl salicylate and camphor content in 8h, good stability. 2.8 Test repeatability precision of this product with the number of samples of 5 copies, according to The RSD = 0.70%, (n = 6) showed good reproducibility of the method. Recovery test by adding 2.9 sample recovery method,[link widoczny dla zalogowanych], known content of samples taken 8, precision reference substance by adding camphor and methyl salicylate reference substance, press the The average recovery was 98.35%,[link widoczny dla zalogowanych], I1SD = 0.60%, (n = 5); methyl salicylate, the average recovery was l02.72%, RSD = 1.20%, 14 Huangshi Institute of Technology 2006 cylinder (n = 5); showed good recovery of the method is feasible. 3.0 Determination of content of the sample taken 5 batches musk strong bone paste, prepared by the test solution were determined under the method (gas chromatography shown in Figure 2), calculated according to the standard two camphor and musk strong bone paste in water Yang acid methyl ester content, the results shown in Table. Table 5 batches of musk strong bone paste of camphor and methyl salicylate assay results 3 Results and discussion 3.1 musk essential oil is anti-inflammatory analgesic effect of strong bone paste the main active fraction, methyl salicylate and camphor essential oil is The main ingredient, so selected as the evaluation of methyl salicylate and camphor cream musk strong bone quality detection index. 3.2 In this paper, manual injection, the needle retaining time and temperature and other factors on the impact of sample size, so that precise control of injection volume Buyi, the standard equation Kejian standard curve has not been the origin, so the use of internal standard for quantitative analysis of two-point method make accurate and reliable results. 3.3 The results show that the two points taken by the determination of the internal standard methyl salicylate and camphor content method is simple, while the 5 different groups of samples are measured methyl salicylate and camphor in there significant differences,[link widoczny dla zalogowanych], but the content of the measured theoretical values calculated with the difference between feeding a larger, possibly with the production process of bake temperature and time control of the. Figure 1 camphor, methyl salicylate, standard, and internal standard gas chromatography Figure 2 samples and internal standard by gas chromatogram
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