defgf67ay
Dołączył: 29 Paź 2010
Posty: 863
Przeczytał: 0 tematów
Ostrzeżeń: 0/5
Skąd: England
|
 Wysłany: Wto 18:17, 15 Lut 2011 Temat postu: moncler outlet Freezing temperature on the recover |
 |
|
Freezing temperature on the recovery of spermatogonia in mice results
Recovery rate; and should not be used as 4 ℃ Received Date :2000-10-16 Author: Lilian Jun (1963 a), female, Hunan Lingling County. In the PhD. Mainly engaged in the embryo Cytology. lO6 Yunnan Agricultural University No. l6 volume recovery of mouse spermatogonia temperature. Table 1 at different temperatures the recovery rate of cell Tab. 1Recoveryratesofspermatogoniaindifferenttemperatures% 3 to discuss a variety of cells are currently the best kept rewarming rate generally faster and more complex every minute over 25-5O ℃ temperature. Due to heavy ice in a knot above 50 ℃ for faster, so the slow thaw is often caused due to cell death re-crystallization, rapid recrystallization temperature can minimize the number of transistors,[link widoczny dla zalogowanych], but also allows cells in a high concentration of solute in the shortest time to smell the reduction to reduce the cell's In this study,[link widoczny dla zalogowanych], the recovery at 4 ℃, the recovery of cyberspace is about 40 ~ 50min, the recovery rate of about 4-5 ~ C / rain,[link widoczny dla zalogowanych], the recovery rate was 29.7%, indicating that the reduced speed of recovery and prolong the recovery time, cell death increased the recovery rate has dropped; and 37 ℃, 67 ℃ and 97 ℃, the recovery time of less than 2min, respectively,[link widoczny dla zalogowanych], the recovery rate of up to 100-150 ~ C / min, 200 ~ 250 ~ C / min and 300-400 ~ C / min, the recovery rate of close to 90%. The results show that the low recovery of spermatogonia negative, while the temperature does not reduce the recovery of at least the recovery rate of spermatogonia. Then from the practical application ,37-67 ℃ recovery of cells more practical. Cells in 67 ℃, 97 it does not do it imply that such cells can tolerate high temperatures, due to the small size makes the thermal conductivity of frozen pipes frozen thawed material and energy in a relatively short period of time,[link widoczny dla zalogowanych], which requires the volume of frozen material can not be too large, otherwise when the external temperature will cause uneven recovery (such as surface temperatures rise higher and not thawed internal) and lead to cell death. Experimental results show that between 37 ~ 97 ℃ recovery lmL tubule spermatogonia cells can obtain a higher recovery rate, which for the recovery of spermatogonial cells frozen to provide a basis for the temperature. [
,],[More articles related to topics：
moncler sito ufficiale Wild cultivated Dioscorea p
[link widoczny dla zalogowanych]
[link widoczny dla zalogowanych]
Post został pochwalony 0 razy
|
|
