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Ig in serum of patients with liver fluke and the determination of C3
1.1.2 control of drug serum of Jiamusi city in Heilongjiang province, Blood of the blood donors, a total of 36, basically the same age and gender study group. 1.2 Methods 1.2.1IgE double antibody sandwich ELISA test to detect the sample. IgE content. Experiments, each of sample wells 200> 1. First to 1O '/ / ml concentration of goat anti-human IgE, IgG coated plate part,[link widoczny dla zalogowanych], the refrigerator at 4 ℃ overnight. Sin plate, add 24 * Third Military Medical University (ActaAcademiaeMedicinaeCPAPF) Vo]. 9No. 1 into 1; 20 diluted serum samples were seized and different concentrations of lgE diluted working standard, 37 ℃ 2h. Washer substrate solution was added three times,[link widoczny dla zalogowanych], 37 ℃ 30min. Finally, each hole by adding 2M sulfuric acid 50 to 1 to stop the reaction, then the wavelength is 492nm in microplate reader on the colorimetric determination of OD values. Operation, were seized each concentration of IgE in serum and work standards for each are parallel respectively with two holes and four experiments. After the experiment,[link widoczny dla zalogowanych], whichever is the mean OD, and then work according to different standards of IgE concentration standard curve drawn samples detected the corresponding total IgE content. 1.2.2IgG, IgA, IgM, and ca were detected by single immunodiffusion method,[link widoczny dla zalogowanych], diffusion panels and working standards by the Ministry of Health,[link widoczny dla zalogowanych], Beijing Institute of Biological Products manufacturing, specific steps according to instructions. 1.2.3 Statistical Methods to £ test experimental data for processing. 2 results of 2.136 cases of liver fluke disease total IgE, IgG, IgA, IgM and C test results (table) with atlas of 36 patients with serum liver fluke down IgE, IgG, IgM and IgA serum test results of liver fluke disease Ig content in addition to the four IgA were significantly higher than that of blood donors outside the detection value (P <O.01). 2.2 The results of complement C patients with serum liver fluke C. Level (1342.5 ± 467.9mg/m1) was significantly lower than the detection value of the control group (1845.0 ± 689.0mg/m1), significant difference (P <0.01). 3 Discussion Kojima and other confirmed by passive cutaneous anaphylaxis in rabbits infected with liver fluke can produce antibody response to claims that such antibodies as early as the third week after infection appears in the serum, and its physical and chemical properties similar to human IgE. Zhou Zheng Ren and other allergens through the disk of radioactive assay to detect serum liver fluke disease in the presence of specific IgE. Our results also showed that human liver fluke infection, total IgE were significantly higher than normal blood donors. Since the human liver fluke infection was significantly increased after the trend of total IgE, indicating that patients with type 1 hypersensitivity may occur, the experiment for liver fluke disease skin test provides a theoretical basis. The results also showed that liver fluke infection after human IgG and IgM in serum were significantly increased, while no change in IgA. This is Zhou Yugui further study. In general, human serum complement were relatively stable, only the risk of certain diseases, total serum complement levels or the composition may occur before the change. clonorchiasis patients c serum complement levels were significantly lower than the normal control group, We believe that CIC may be associated with increased levels of serum and liver function impairment. As to the exact mechanism and clinical significance remains to be further explored. 4
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