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 Wysłany: Sob 9:21, 15 Sty 2011 Temat postu: Galactomyces geotrichum Y25 Lipase Production Opti |
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Galactomyces geotrichum Y25 Optimization of lipase production
ime (B,[link widoczny dla zalogowanych], b) soybeanflourVS. culturetime, fixedcenterlevelofcornsteepliquor (c,[link widoczny dla zalogowanych], c) cornsteepliquorVS. culturetime, fixedcenterlevelofsoybeanflour,[link widoczny dla zalogowanych], Lu Jie ● disk a rIg. n) \ slug poison a rIg. f1) / slug poison an r1. f1) / Jie Yi a r1. rD / slug poison a _rIg. f1) / slug poison May 2007 Yan Jinyong, etc.: GalactomycesgeotrichumY25 Lipase Production Optimization '51 * 3 Conclusion optimized lipase enzyme activity up to 34.65U/mL,[link widoczny dla zalogowanych], compared to the initial fermentation activity 9.6U/mL improve the 3.6l times. Visible, Plackett-Burman method and response surface method is an effective microbial fermentation method can quickly and efficiently from a number of factors affect the lipase fermentation a significant factor in the selection and to achieve its level of optimization, greatly improve the enzyme production volume. Optimized G. geotri-chumY25 best medium lipase fermentation liquid composition (mass fraction): 2.51% soybean meal,[link widoczny dla zalogowanych], corn steep liquor 2.12%, sucrose 1.0%, (NH4) 2SO40.1%, K2HPO40.2% , MgSO4 · 7H2O0.01%, olive oil, the volume fraction of 1%. Culture conditions: fermentation temperature is 28, medium with initial pH6.0, shaking speed 220r/min, training 101.95h.
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