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 Wysłany: Nie 11:45, 30 Sty 2011 Temat postu: Department of Newhall navel orange and its RAPD an |
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Department of Newhall navel orange and its RAPD analysis of mutant
In the subsequent PCR amplification. 1.2.2RAPD reaction Shanghai Health workers use random primers, S-60. 25L reaction system: 12.5L2 × PCRbuffer, 0.5LTaq enzyme, 2.5L primer, 8.5LddH2O, 1 belly L template DNA. Reaction program was: 94 ℃ for denaturing 2min, 94 ℃ denaturation 0.5min, 35.2 ℃ annealing 0.5min, 72 ℃ extension 1.5min, 38 cycles, the last 1 to 72 ℃ for 7min,[link widoczny dla zalogowanych], 4 ℃ preservation. 8 belly LPCR products obtained in the 1.0 agarose gel (withEB) electrophoresis 40min, voltage 130V, after SYNGENE gel imaging analysis system were observed and photographed. 1.2.3 Data analysis of the amplified diagrams SYNGENE gel imaging analysis system,[link widoczny dla zalogowanych], amplified by a band (1), no (O) records, the use of statistical analysis software NTSYSpc2.1 cluster analysis, drawing the dendrogram. 2 Results and analysis 2.1DNA amplified from 60 random primers, 12 primers, two of the tested varieties (lines) for RAPD analysis, 91 were amplified with an average of 7 per primer .6 bands. Among them, a polymorphic, polymorphism ratio of 1.10. Primers, $ 226 received the highest proportion of polymorphic bands, 12.5, and the resulting band size of 300 ~ 2250bp (Table). Primers, $ 226, $ 228 amplification profiles shown in Figure 1. Figure 1 shows, 2 navel orange varieties (lines) between the differences,[link widoczny dla zalogowanych], but less polymorphic. Only $ 2,262,000 in the molecular weight of primers were found to have specific bands. 2.2 The results of cluster analysis based on statistical analysis of DNA amplification, two orange products $ 226S228M the marker, 1 for the long round of Newhall navel orange, Newhall navel orange 2 Figure 1 for the general $ 226 and $ 228 to 2 navel orange cultivars (lines) of the amplification results of Fig. 1Theamplificationresultofprimer $ 226andprimer $ 228 table of 12 RAPD primers 2 navel orange varieties (lines) on the amplification results TableTheamplificationresultofNewhallnavelorangeanditsvariantlinebyusing12RAPDprimers oblong Newhall Newhall ------] IIIl ------- 0.00o . OluuzGENETICDISTANCE Figure 22 navel orange cultivars (lines) of the RAPD dendrogram Fig. 2TheclusterdendrogramofNewhallnavelorangevarie-tyanditsvariantline species (lines) between the genetic distance (GD) is 0.0055. 2 varieties (lines) between the cluster tree shown in Figure 2.3 Conclusion and discussion 1) RAPD analysis showed that the oblong Navel Orange Navel Orange and the level of the DNA is different, but the genetic distance between them (GD) is extremely small, only 0.0055. 2) used in the study of two varieties (lines ) genetic distance between the small, non-higher proportion of polymorphic bands (98.90), RAPD analysis of specific bands of small, both in morphology and growth habit differences, and smaller is the same. 3) RAPD analysis showed that the oblong shape of Newhall navel orange by the gene-specific control, rather than by the variation caused by environmental impact. This variety of Newhall navel orange oblong further promotion and application of assessment and laid the foundation. [
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