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 Wysłany: Wto 14:01, 08 Mar 2011 Temat postu: adidas scarpe iin gln luje jgv |
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Schistosoma japonicum adult female cDNA library screening serum antigen
alewormgender-specificgeneisofgreatattraction. [Keywords] Schistosomajaponicum; femaleworm; cDNAlibrary; immunoscreening; sequenceanalysis praziquantel, albendazole and allicin in vitro the role of anti-nematode Xu Zhengmin hidden tube, Tao Yongping, aftermath, Li Ping, Zhang Zhiyong (1. Xiangfan Vocational and Technical School of Medicine Branch, Xiangfan 441021, China; 2. Xiangfan First People's Hospital) 【Key Words】 R383.1 Document code】 【B】 【article number 100 BU 6627 (2004) 01-0041-01 praziquantel and Albendazole is a broad-spectrum and low toxicity anti-worm drug, widely used in trematodes, nematodes, and the treatment of taeniasis of allicin on certain intestinal protozoa have a certain role _1]. Hide tubular nematodes (Syphaciaobuelata) is a rodent parasite, in order to observe the praziquantel, albendazole and allicin in vitro resistance to hide the role of tube worm, of the experimentally observed, the results reported below. Materials and methods Preparation of a medium 200ml and 50ml with HankS fluid containing 1640 bovine serum mix, and add penicillin 200U/ml and streptomycin 200tzg/ml,[link widoczny dla zalogowanych], pH7.4, was red. -2 Drugs and use of praziquantel and albendazole, respectively, in Shanghai and Hubei Pharmaceutical Factory Pharmaceutical November, garlic extract solution for use. The culture medium were divided into three 150ml bottles, 2 bottles were joined by i0btg/ml praziquantel, albendazole, a bottle of garlic solution (30), as the experimental group. 100ml left without medication as control group. Isolation and culture of parasites were killed 3 mice, dissecting intestine, home saline, picked with a cotton swab tube worm hidden in control medium at 37 ℃, washed 2 times, and then select the motility of the parasites, were placed with 3 Experimental bottles of different drugs and a drug-free control flask, bottle article 1O, 37 ℃ incubator culture, 24h 1 medium replacement, sterile operation, a continuous observation of 3d. Observation methods 4.1 Observation 4 activity were observed visually and recorded parasites experimental group and control group activity level. 4.2 Observation of the substrate to retain each culture medium and microscopic examination of its replacement of the substrate. 4.3 3d morphology after culture with 5 formaldehyde fixed parasites, low magnification observation of morphology. 4.4 histochemistry to show glycogen PAS method, cobalt sulfide showed acid phosphatase, calcium, alkaline phosphatase assay showed that a cobalt acid enzymes. * Summary of the results of an activity observed after 24h culture visible worms in each experimental group retardation. Active control group, 48h after the worm close to the bottom and the experimental group; microscopy, parasites can be seen in all experimental groups around the upper half swing the ball up and down the esophagus stretching, 72h after the larvae were inactive. Control group, almost no change in parasite viability. 2 medium substrate observed in control group after 48h culture medium substrate, the visible discharge of the egg (Figure 1), 72h still visible eggs. Eggs seen in each experimental group 24h, 48h after the small quantity of eggs, 72h of no eggs. 3 Light microscopy morphological parasite control group showed a linear,[link widoczny dla zalogowanych], full of natural body, the female was spindle, straight and tapered tail, the ventral male tail curling. Clear internal structure, the first wing, the esophagus, esophageal ball well, with a distinguishing characteristic (Figure 2), female tail tube is clearly visible (Figure 3). Deformed larvae were observed in praziquantel. Was 9O degree bend. Disappearance of the first wing (Figure 4.5), esophagus, esophagus ball deformation, structural fuzzy, raised different degrees angle cortex (Fig. 4 to 7). Deformation of the first wing of albendazole group of parasites. Distorted, esophagus, esophageal ball, intestinal support structure fuzzy (Figure  , bent tail, the internal structure is unclear,[link widoczny dla zalogowanych], we can see a lot of eggs (Fig. 9). Worms form allicin group did not change, but the first wing, esophagus, esophageal Sphere obscure (Fig. 10),[link widoczny dla zalogowanych], the tail tube structure is not clear,[link widoczny dla zalogowanych], the color black (Figure 11), and the cortex shows a small convex parasites onwards (Figure 12). (This figure see back cover) 4 histochemistry of glycogen check the strength of positive, according to judge the depth of red coloring, glycogen content in the control group more deeply colored, PAS positive; albendazole praziquantel group the light color, glycogen decreased; allicin was no change. Acid phosphatase, alkaline phosphatase according to the degree of difficulty of a black judge the strength of activity, three experimental and control groups of acid phosphatase, alkaline phosphatase activity does not seem different. (The switch to 48 pages)
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